About laboratory

	Head of the Laboratory - Doctor (physical and mathematical sciences), Prof. Zasedatelev Alexander Sergeevich. (Deputy Director of EIMB RAS and Head of the Chair of Molecular Biophysics of Moscow Institute of Physics and Technology) tel.: 8(499)135-98-00
		Deputy Head of the laboratory PhD (biology) Mikhailovich Vladimir Mikhailovich tel.: 8(499)135-11-77
	Group of analysis of pathogenic bacteria and viruses PhD (biology) Gryadunov Dmitry Aleksandrovich tel.: 8(499)135-98-46
		Group of human genome analysis PhD (biology) Nasedkina Tatiana Vasilievna tel.: 8(499)135-62-59
	Protein microchips group PhD (chemistry) Rubina Alla Yurievna tel. 8(499)135-99-80

• Study of structure and function of biological molecules and biomolecular complexes:

  - study of specificity of DNA-DNA, DNA-ligand, and DNA-protein interactions using "generic" microchips containing complete set of all possible hexamerous oligonucleotide sequences (4⁶ = 4096 oligonucleotides). Generic microchips permit one to carry out multiparameter analysis of thermodynamic binding characteristics;
  - study of specificity of protein-NA, protein-ligand, and protein-protein interactions using protein microchips; multiparameter analysis of thermodynamic binding characteristics;
  - analysis of genomes of pathogenic bacteria and viruses and determination of their resistance against different drugs using biological microchips;
  - investigation of diffusion and hybridization processes in gels for the molecules (DNA, proteins or protein fragments) that form specific complexes with gel-immobilized oligonucleotides or proteins.
  

• Analysis of mechanisms for realization of genetic information:

  - analysis of human genome polymorphism and mutations which determine susceptibility to some oncological or hereditary diseases and individual sensitivity to some types of drug therapy;
  

• Development of biochips for fundamental studies:

  - development of "generic" biochips containing complete set of all possible hexamerous oligonucleotide sequences in 4096 elements for multiparameter analysis of thermodynamic characteristics of DNA-DNA, DNA-ligand, and DNA-protein binding specificity;
  - development of protein and glycan biochips for multiparameter analysis of thermodynamic characteristics of protein-NA, protein-ligand, protein-protein, and protein-glycan binding specificity.
  

• Development of biochips for medical diagnostics and DNA database formation:

  - development of oligonucleotide biochips for the detection and genotyping of pathogenic bacteria and viruses and for the analysis of their resistance to drugs;
  - development of oligonucleotide biochips for the analysis of genetic susceptibility to some oncological or hereditary diseases and individual sensitivity to some types of drug therapy;
  - development of biochips for the typing of chromosomal translocations and mutations at oncological diseases;
  - development of protein biochips for the quantitative assay of tumor markers of protein nature in human serum;
  - development of protein biochips for the detection and quantitative assay of plant and bacterial toxins;
  - development of biochips for the genetic identification of person for forensic medical examinations and DNA database formation.
  

• Creation of instrumental and analytical bases for biochip technology:

  - construction of biochip analyzers with necessary software for the application of biochips in fundamental research and medical diagnostics;
  - construction of automated device on the basis of removable cartridges for the isolation of microamounts of nucleic acids from biological samples.
  

• Development of instruments for mass biochip production:

  - creation of pilot-scale production of biochips and biochip-based test-systems for subsequent certification and application in medical diagnostics;
  - design and development work within the framework of the State innovation program "Development and mass application of new technologies for diagnosing of socially important diseases on the basis of molecular methods of multiparameter analysis".
  

• Training and transfer of biochip technology to interested scientific and medical organizations:

  - support of scientific and applied studies of the interested RAS and RAMS institutions, medical establishments, and other organizations in the Center of Collective Use of Biological Microchips, which was created on the basis of the Laboratory of Biological Microchips.
  

• Generic biochip containing all possible hexameric deoxyribonucleotide sequences in 4096 cells has been created. This biochip was used to determine sequence-specificity of binding of HU-8, p50, λ-Cro V55C regulatory proteins and Hochst-33258 dye with all hexanucleotide DNA sequences.
• The technology for protein biochip manufacturing has been developed. Protein biochips permit carrying out multiparameter analysis of protein-protein and protein-ligand interactions. It has been demonstrated that protein molecules, including enzymes, maintain their biological activity upon immobilization in biochip gel elements.
• The method for carrying out real-time PCR in biochip gel elements has been developed;
• Biochip nanotechnology has been developed and patented. The technology permits carrying out rapid analysis of socially important infections, oncological, cardiovascular, and hereditary diseases and revealing extremely important infections and biotoxins; it is now introducing into medical diagnostics.

• causative agent of TUBERCULOSIS and drug-resistant forms of tuberculosis. The assay time is 18 hours instead of 6-10 weeks (when using the standard method). The method has no analogs; it is certified for medical application in Russia and is used in a series of anti-tuberculosis centers;
• chromosomal translocations (8 types) at LEUCOSIS. The method is certified for medical application in Russia. It is now used in Russia and tested in the USA and Brazil;
• genetic susceptibility to a series of oncological and hereditary diseases and individual sensitivity to some types of therapy;
• causative agents of HIV, hepatitis B and C (22 subtypes);
• causative agents of VARIOLA, animal smallpox, chickenpox, herpes simplex (2 types);
• causative agents of ANTHRAX;
• TUMOR MARKERS of protein type;
• the presence of some types of BIOTOXINS (16 types);
• causative agents of neonatal infections;
• typeA INFLUENZA virus (30 subtypes) including birds flu H5N1;
• genetic identity of a person when carrying out forensic medical examinations and DNA database formation.